• 我們歡迎有學習熱情 能跑 能跳 能思考 能抗壓的大專生加入主題計畫!

    Great fun in working with plants

  • Education and Academic Life

    Expertise: Plant molecular and cell biology; Plant physiology; Plant molecular transport; Plant biotechnology

    2014 Assistant Professor, Institute of Bioinformatics & Structural Biology, National Tsing Hua University

    2008 - 2014 Postdoc, Agricultural Biotechnology Research Center (ABRC), Academia Sinica

    2008 Ph.D., Center for Plant Molecular Biology (ZMBP), Department of Plant Physiology, Universität Tübingen, Germany

    2003 M.S., Institute of Microbiology & Immunology, National Yang-Ming University

    2001 B.S., Department of Biotechnology & Laboratory Science in Medicine, National Yang-Ming University

  • Research Intersts

    Plants as autotrophic organisms capture and convert the solar energy, and store or distribute it in various forms of biomolecules to provide us food, medicine, and materials. As their health and productivity impose a great impact on nearly every aspect of human activities, plant science becomes an important and fascinating research field in perspective of achieving sustainability in agriculture and environment. In our lab, we are interested in identifying the molecular mechanisms by which plants respond and adapt to a variety of abiotic stresses as well as developing tools or techniques to address fundamental questions in plant biology through innovation. Our research is currently focused on the following topics using Arabidopsis as the model organism:

    • Developing new molecular tools for protein-protein interaction screening in planta

    Searching for novel protein-protein interactions becomes a major and challenging task to elucidate protein functions as well as its regulatory network. Although in planta screening methods, which allow for proper protein modifications and physiologically relevant interaction environment, are potentially more reliable in terms of minimizing unspecific behaviors observed in heterologous systems, only few attempts have been made for establishing library-scale screens in planta. Our research project is aimed to develop new molecular tools for high-throughput screens of protein-protein interactions in planta, particularly suitable for identification of transporter-interacting proteins.

    Arabidopsis vacuolar Pi transporter  PHT5
    • Mechanism of inorganic phosphate (Pi) transport across the tonoplast (vacuolar membrane) in plants

    Phosphorus (P) availability in the form of inorganic phosphate (Pi) acquired by plant is limited. To maintain cellular Pi homeostasis, plants utilize a series of regulatory mechanisms, including coordination of discrete Pi transport activities across biomembranes for compartmentalization. The vacuole serves as a primary organelle for Pi storage and remobilization. Despite the critical role of vacuolar Pi (vac-Pi) in buffering the cytoplasmic Pi (cyt-Pi) level against fluctuations caused by variable external Pi supply and metabolic activities, the long-sought vacuolar Pi transporters remain unidentified. Currently, we are characterizing the genes encoding putative tonoplast-localized Pi transporters that contribute to the maintenance of cyt-Pi homeostasis.

  • Awards and honors

    2001 National Certificate of Qualification of Laboratory Science in Medicine (國家醫檢師執照)

    2004 – 2005 Deutsche Forschungsgemeinschaft (DFG) Promotionsstipendium

    2006 – 2008 Wissenschaftliche Mitarbeiter (zur Promotion an Tübingen Universität)

    2009 – 2012 Academia Sinica Postdoctoral Fellowship

    2009 Annual Best Poster Award by ABRC, Academia Sinica

    2010 Annual Best Poster Award by ABRC, Academia Sinica

    2011 Young Scientist Award Second Prize (Plant Section), the Society of Experimental Biology

    2012 National Science Council Award for Postdoctoral Researcher (國科會博士後研究人員學術著作獎)

    2013 Travel Grant Award by ABRC, Academia Sinica

    2013 Oct Technical Training - vacuole isolation and transport assay in Prof. Enrico Martinoia’s lab, Institute of Plant Biology, University of Zürich, Switzerland

    2014 Invited speaker 中華植物學會暨創新植物及生物科技研討會

    2016 楊祥發院士傑出農業科學年輕學者獎

    2017 國立清華大學第三十屆新進人員研究獎

    2017 Invited speaker 台灣生物化學及分子生物學學會

  • Publications (2008-2016)

    Liu, T.-Y.*, Huang, T.-K.*, Yang, S.-Y.*, Hong, Y.-T., Huang, S.-M., Wang, F.-N., Chiang, S.-F., Tsai, S.-Y., Lu, W.-C., and Chiou, T.-J. (2016). Identification of plant vacuolar transporters mediating phosphate storage. Nature Communications 7:11095 (*equal contribution; SCI, 5-year IF: 11.90) The 9th楊祥發院士傑出農業科學年輕學者獎

     

    Liu, T.-Y., Lin, W.-Y., Huang, T.-K., and Chiou, T.-J. (2014). MicroRNA-mediated surveillance of phosphate transporter on the move. Trends in Plant Science 19, 647-655. (SCI, 5-year IF:14.67)

     

    Huang, T.-K., Han, C.-L., Lin, S.-I, Chen, Y.-J., Tsai, Y.-C., Chen, Y-R., Chen, J.-W., Lin, W.-Y., Chen, P.-M., Liu, T.-Y., Chen, Y.-S., Sun, C-M., and Chiou, T.-J. (2013). Identification of downstream components of PHO2 by quantitative membrane proteomics in Arabidopsis. Plant Cell 25, 4044-4060. (SCI, 5-year IF: 10.53)

     

    Liu, T.-Y., Huang, T.-K., Tseng, C.-Y., Lai, Y.-S., Lin, S.-I, Lin, W.-Y., Chen, J.-W., and Chiou, T.-J. (2012). PHO2-dependent degradation of PHO1 modulates phosphate homeostasis in Arabidopsis. Plant Cell 24, 2168-2183. (SCI, 5-year IF: 10.53) 國科會101年度博士後研究人員學術著作獎

     

    Liu, T.-Y., Aung, K., Tseng, C.-Y., Chang, T.-Y., Cheng, Y.-S., and Chiou, T.-J. (2011). Vacuolar Ca2+/H+ transport activity is required for systemic phosphate homeostasis involving shoot-to-root phosphate signaling in Arabidopsis. Plant Physiology 156, 1176-1189. (SCI, 5-year IF: 8.03)

     

    Dettmer, J.*, Liu, T.-Y.*, and Schumacher, K. (2010). Functional analysis of Arabidopsis V-ATPase subunit VHA-E isoforms. European Journal of Cell Biology 89, 152-156. (*equal contribution; SCI, 5-year IF: 3.65)

     

    Liu, T.-Y., Chang, C.-Y., and Chiou, T.-J. (2009). The long-distance signaling of mineral macronutrients. Current Opinion in Plant Biology 12, 312-319. (SCI, 5-year IF: 9.20)

     

    Neubert, C., Graham, L.A., Black-Maier, E.W., Coonrod, E.M., Liu, T.-Y., Stierhof, Y.-D., Seidel, T., Stevens, T.H., and Schumacher, K. (2008). Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p. Traffic 9, 1618-1628. (SCI, 5-year IF: 4.34)

     

    Brüx, A.*, Liu, T.-Y.*, Krebs, M., Stierhof, Y.-D., Lohmann, J.U., Miersch, O., Wasternack, C., and Schumacher, K. (2008). Reduced V-ATPase activity in the trans-Golgi network causes oxylipin-dependent hypocotyl growth inhibition in Arabidopsis. Plant Cell 20, 1088-1100. (*equal contribution; SCI, 5-year IF: 10.53)

    Grant Support

    2016- 2019 科技部自然司專題研究計畫

    2014- 2016 科技部生科司專題研究計畫

    2015- 2016 科技部大專生專題研究計畫 (林柏成同學)

    2015- 2016 清大生科院大專生專題研究計畫 (吳佩諭同學)

    2016- 2017 科技部大專生專題研究計畫 (戴禎逸同學/榮獲105年度科技部大專學生研究計畫研究創作獎)

    2017- 2018 科技部大專生專題研究計畫 (龍惠方同學)

    Lectures

    生命科學二

    植物生理學

    生命科學之模式生物

    植物分子生物技術特論

    阿拉伯芥模式生物研究特論

    植物科學新聞

    植物自噬作用特論

    碩士班書報討論

    Service

    清大生科院豐盛生命師生團契輔導老師

    清交研究生必勝研習會召集人

    清大“勇於教學”教師社群召集人

  • LAB members

    林柏成

    生資所碩班

    推甄逕行錄取/獲104學年度沈巨塵先生清華獎學金(碩班)

    吳佩諭

    生資所碩班

    推甄錄取

    戴禎逸

    生資所碩班

    推甄逕行錄取/獲106學年度沈巨塵先生清華獎學金(碩班)

    郭祐豪

    生資所碩班

    龍惠方

    生科系

    楊承翰

    生科系

    林桔安

    生科系

    林莉晏

    生科系

    周玟醇 (研究助理)

    快樂學習專區

    2008 探索植物基因 改善糧食問題–米雪‧戴森博士

    https://scitechvista.most.gov.tw/zh-tw/Articles/C/0/12/10/1/1182.htm

    2009 磷礦耗竭的危機 (科學人) David A. Vaccari 翻譯/林筱雯
    http://sa.ylib.com/MagCont.aspx?Unit=featurearticles&id=1414
    2011 基因改造植物面面觀 賀端華 | 中央研究院院士
    https://scitechvista.most.gov.tw/zh-tw/lectures/c/0/10/10/41.htm
    2012 植物界的最佳代言人 林彩雲 | 清華大學生物資訊與結構生物研究所教授

    https://scitechvista.most.gov.tw/zh-tw/Articles/C/0/1/10/1/1673.htm

    2015 伊波拉救星:菸草疫苗

    http://www.30.com.tw/article_content_29055.html

    2016 Educational Resources for plant biology by ASPB

    http://blog.aspb.org/2016/09/06/educational-resources-for-plant-biology-longer-accessible-videos-ted-talks-ibiology-and-gatsby-plants-summer-school-lectures/

  • Where we are?